Phytochemical estimation, antioxidant and antimicrobial properties of Pleocaulus sessilis: an endemic plant from Western Ghats of India

Sujata V. Patil1, Rahul P. Mane1, P. V. Anbhule1,2 and V. B. Shimpale1,3* M. B. Deshmukh1,2
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The phytochemical constituents antioxidant and antimicrobial activities of extracts of Pleocaulus
sessilis using different solvents were evaluated. The dried powdered leaves of Pleocaulus sessilis
were extracted with acetone, ethanol and methanol. All extracts were analyzed by high performance
liquid chromatography (HPLC) to estimate the compound content. The phytochemicals were
estimated according to the standard methods.1,1-diphenyl-2-picrylhydrazyl,Hydrogen peroxide and
reducing power assay were used to determine in vitro antioxidant and antimicrobial activities and
were performed using standard protocol. Phytochemical analysis of extracts showed presence of
main group phytochemicals such as phenols, flavonoids, saponins and tannins. Methanolic extract
was found to contain the highest phenolic and flavonoid content. In vitro antioxidant activities and
antimicrobial activities of all extracts were found to be good. On the basis of the results obtained,
all the varied extracts of Pleocaulus sessilis seems to be the most capable species for further
exploration in order to identify the chemical ingredients responsible for their activity.
Keywords: flavonoid content, phenolic content, RP- HPLC, Antioxidant activity, Pleocaulus
sessilis.