Study of the Cytotoxic effect of a Novel Ampicillin ZnO Nanocomposite Against Myeloma Cell Lines

Zahira S. Tawfik,a Abdelfattah M. Badawi,b Ibrahim Y. Abdel-Ghany,C Zizi I. Abdeen* b
Page no. 64-72

In the present work, the effect of the standard ampicillin, its ZnO nanocomposite or ZnO nanoparticles on myeloma cell line SP2OR in vitro was studied.  Variable doses ranging from 10 to 100 µg /ml from the three compounds were used.  The results indicated that the viability (%) of myeloma cells was not influenced by increasing doses (10-100 µg/ml) of ampicillin during the time of experiment.  On the other hand the ampicillin ZnO nanocompoiste showed a significant effect on the viability of the myeloma cells.  The viability was decreased by increasing doses from 10 to 100 µg /ml until reached its maximum at the third days of incubation of the cells with the ampicillin ZnO nanocomposite (zero) at dose 100 µg/ml.  The results of the effect of Zinc Oxide on the myeloma cell lines at the same doses indicated a significant decrease by increasing doses which reached its maximum of viability of 2.7% at 100 µg/ml and it was less potential than the corresponding ampicillin ZnO nanocomposite which reached its maximum viability of zero at the dose.

Keywords: Ampicillin, ZnO nanocomposite, ZnO nanoparticles, myeloma cell line